Euphorbia hirta Linn. [Euphorbiaceae; Sp. Pl. (1753)] also called as asthma plant. Traditionally, it is used in the treatment of tumors, fever, asthma, bronchitis, chest congestion, etc. Objective of the study was to evaluate cytotoxicity of methanolic extract of E. hirta using HCT-15 and Vero cell line. MTT assay was used to performed cytotoxicity study of methanolic extract of E. hirta. Doxorubicin was considered as standard drug. Concentrations of extract and doxorubicin were 0.05-1000 µg/ml used. The 50% cell growth inhibition (IC50) of methanolic extract of E. hirta and doxorubicin was 576.82 µg/ml and 460.13 µg/ml against the HCT-15 cell line and 1375.22 µg/ml and 2392.71 µg/ml against vero cell line respectively. Methanolic extract of E. hirta and doxorubicin was effective cytotoxic drugs against HCT-15 cell line and was safe to normal vero cell line. It is due to the extraction of active compounds in the methanolic extract. These are may be new unknown entities or either constituent reported in the earlier studies. Chemical analysis of the extract is in under pipeline. First time author has reported these studies on E. hirta using HCT-15 and vero cell line by MTT assay. This effect can be studied using an allograft and xenograft experimental technique which is not evaluated yet on this plant. Therefore, it is suggested that assessment of active constituents and clinical evaluation of methanolic extract of E. hirta would give a positive lead in the successful treatment of tumors or cancers.
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